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Förslaget inkom 2003-06-26

Prokaryotic cell biology: The role of actin-like cytoskeletons in bacteria

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It has been believed that bacteria lack internal cytoskeletons and that their cell shapes are determined by their rigid cell walls. This view was recently overturned when an actin-like cytoskeleton was discovered in bacteria (Jones et al., 2001, Cell 104: 913-922; van den Ent et al., 2001, Nature 413:39-44). Two genes encoding actin-like proteins were found to have profound influence on the shape and integrity of cells in the Gram-positive bacterium Bacillus subtilis, but the genes involved are widespread among prokaryotes. The proteins assembled into helical filaments, stretching along the entire cell or parts of it. The study of these new cytoskeletal elements has many implications for the evolution and function of eukaryotic actin, and for understanding the infrastructure and function of simple bacterial cells. It also opens novel possibilities for developing antibiotics that would target these structures.

In this project, we will examine the roles of such actin-like proteins in the model organism Streptomyces coelicolor, a filamentous bacterium, which for several reasons is particularly well suited for these analyses. In particular, it enables us to study the involvement of actin-like proteins in polar growth. This organism contains three homologues of the actin-like proteins, and the function of these will be examined. The formation of subcellular structures by these proteins will be examined by genetically engineering epitope tags onto the proteins, and to detect them in vivo by immunofluorescence microscopy. Then, immunoprecipitation will be used to identify proteins interacting with these cytoskeletal structures.


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