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Förslaget inkom 2010-04-07

Perfusion by ATF filtration system in Chinese Hamster Ovary cell cultivation

OBS! ANSÖKNINGSTIDEN FÖR DETTA EXJOBB HAR LÖPT UT.
The cultivation of Chinese Hamster Ovary cells, CHO, is widely used in the biopharmaceutical industry for the production of drug proteins. Perfusion is a mode of operation where a continuous replacement of the conditioned medium by fresh medium is operated. It has the advantage of allowing high cell densities and therefore increased higher product titres. This mode of operations is also required for some instable proteins since it allows the immediate storage of the cell-free supernatant containing the product of interest at low temperature where the proteolysis is not active. The ATF filtration device has been designed to perfuse mammalian cell cultivation process and has been recently commercially available.
Our group has developed a bioreactor system perfused with an ATF system and shown cell densities as high as 48 * 10^6 cells/mL using a CHO cell line. The purpose of the present work is to improve the actual perfusion system and improve the knowledge of this system. The work will mainly focus on studying the parameters of the ATF system for optimal perfusion, identifying the limitations, etc. The work will be performed in collaboration with GE Healthcare (Sweden) and the cultivation medium will sponsored by Irvine Scientific (CA, USA).

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