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Förslaget inkom 2004-01-07

Developmental control of cell division in Streptomyces coelicolor

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Bacteria from the genus Streptomyces are major antibiotic producers. This makes their physiology a field of study of immense industrial significance.
The model organism Streptomyces coelicolor undergoes a complex life cycle with at least two types of cell division - vegetative septation and sporulation. Both processes involve formation of a cytokinetic ring by the FtsZ protein, a tubulin homologue that is the main cell division protein in bacteria. The goal of our project is to understand the developmental control of cell division, i.e. what determines where and when sporulation septa are laid down, and what makes this different from vegetative septation.
We have created a translational fusion of ftsZ to the gene for GFP, which allows us to visualise Z ring formation in vivo during vegetative growth and sporulation. A screening system has been set up to identify mutations in ftsZ that specifically disrupt sporulation, while not significantly affecting vegetative growth. So far, one such mutant has been isolated and characterised (Grantcharova et al., Molecular Microbiology, in press). Results suggest that our genetic screen will provide novel information on FtsZ function. This is particularly important because of the application of cell division as a target for antimicrobial drugs.
Some techniques that will be used are random PCR mutagenesis, site-directed PCR mutagenesis, molecular cloning and fluorescence microscopy combined with advanced digital image processing.


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